Product Name:
Human Mitogen Activated Protein Kinase 1 (MAPK1) ELISA Kit
Cat.#:
JL19815
Regulatory Status:
Alternative:
Erk2; Mapk; Prkm1
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
46.88-3000pg/mL
Sensitivity:
25pg/mL
Specificity:
This kit recognizes Human MAPK1 in samples. No significant cross-reactivity or interference between Human MAPK1 and analogues was observed.
Sample Type:
Tissue homogenates;cell lysates and other biological fluids
Assay Procedure:
Research Area:
Neuroscience
Size & Price: 
Product Datasheets
Instruction: 
| MAPK1
Mitogen-activated protein kinase 1 (MAPK 1), also known as ERK2, is an enzyme that is encoded by the MAPK1 gene.The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases.
| Repeatability
Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%| Recovery
The recovery of Human MAPK1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.| Sample Type | Range(%) | Average Recovery(%) |
| Serum(n=8) | 84-101 | 96 |
| Plasma(n=8) | 92-105 | 102 |
| Tissue Homogenates(n=8) | 96-108 | 105 |
| Linearity
Samples were spiked with high concentrations of Human MAPK1 and diluted with Universal Diluent to produce samples with values within the range of the assay.| Serum(n=4) | Plasma(n=4) | Tissue Homogenates(n=4) | ||
| 1 : 2 | Range (%) | 84-95 | 88-96 | 90-110 |
| Average (%) | 91 | 95 | 96 | |
| 1 : 4 | Range (%) | 89-103 | 87-108 | 105-114 |
| Average (%) | 94 | 98 | 108 |
Related Product
Size
Quantity
Price ($)
48T
1
360 / 360
96T
1
460 / 460